兔抗ECH1多克隆抗体

Background:

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Applications:

ELISA, WB, IHC

Name of antibody:

ECH1

Immunogen:

Fusion protein of human ECH1

Full name:

enoyl-CoA hydratase 1

Synonyms:

HPXEL

SwissProt:

Q13011

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human tonsil

IHC Recommend dilution:

50-300

WB Predicted band size:

36 kDa

WB Positive control:

Human fetal liver tissue, Hela, 293T, HepG2 and A549 cell lysates

WB Recommended dilution:

500-2000