小鼠抗SPANXB1单克隆抗体

Background:

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular family member contains an additional 18 nucleotides in its coding region compared to the other family members in the same gene cluster. This family member is also subject to gene copy number variation. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis.

Applications:

WB, IHC

Name of antibody:

SPANXB1

Immunogen:

Fusion protein of human SPANXB1

Full name:

SPANX family member B1

Synonyms:

B1; CT11.2; SPANXB; SPANX-B; SPANXB2; SPANXF1; SPANXF2

SwissProt:

Q9NS25

IHC positive control:

Human gastric stromal tumor tissue and Human spleen cancer

IHC Recommend dilution:

500-1000

WB Predicted band size:

12 KD

WB Positive control:

MDA-MB231 cell lysate

WB Recommended dilution:

500-2000