小鼠抗H2AX(Phospho-Ser139)单克隆抗体

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Applications:

WB, IF

Name of antibody:

H2AX

Immunogen:

Fusion protein of human H2AX(Phospho-Ser139)

Full name:

H2A.X variant histone

Synonyms:

H2A.X; H2A/X; H2AFX

SwissProt:

P16104

WB Predicted band size:

15 kDa

WB Positive control:

3T3 cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

3T3 cells

IF Recommend dilution:

200-400