兔抗ACAP1多克隆抗体

Background:

ACAP1, also designated Centaurin-∫ 1 (CENTB1 or Cnt-∫1), is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP). GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.

Applications:

ELISA, WB, IHC

Name of antibody:

ACAP1

Immunogen:

Fusion protein of human ACAP1

Full name:

ArfGAP with coiled-coil, ankyrin repeat and PH domains 1

Synonyms:

CENTB1

SwissProt:

Q15027

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human thyroid cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

82 kDa

WB Positive control:

Mouse lung tissue lysate

WB Recommended dilution:

200-1000