小鼠抗BRAF单克隆抗体

Background:

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Applications:

WB

Name of antibody:

BRAF

Immunogen:

Fusion protein of human BRAF

Full name:

B-Raf proto-oncogene, serine/threonine kinase

Synonyms:

NS7; B-raf; BRAF1; RAFB1; B-RAF1

SwissProt:

P15056

WB Predicted band size:

84 kDa

WB Positive control:

MDA-MB-468, T47D, MCF7, HCT116, C2C12, 3T3 and Hela cell lysates

WB Recommended dilution:

1000-5000