小鼠抗PPM1D单克隆抗体

Background:

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Applications:

WB

Name of antibody:

PPM1D

Immunogen:

Fusion protein of human PPM1D

Full name:

protein phosphatase, Mg2+/Mn2+ dependent 1D

Synonyms:

WIP1; IDDGIP; PP2C-DELTA

SwissProt:

O15297

WB Predicted band size:

67 KD

WB Positive control:

Jurkat, HEK293, Hela cell lysates

WB Recommended dilution:

500-2000