小鼠抗ATP6V1B2单克隆抗体

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC

Name of antibody:

ATP6V1B2

Immunogen:

Fusion protein of human ATP6V1B2

Full name:

ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)

Synonyms:

HO57; VATB; VPP3; Vma2; ATP6B2; ATP6B1B2

SwissProt:

P21281

IHC positive control:

human breast tissue and carcinoma of human liver tissue; adenocarcinoma of human ovary tissue and adenocarcinoma of human breast tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

57 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000