小鼠抗MDM4单克隆抗体

Background:

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011].

Applications:

WB, IF

Name of antibody:

MDM4

Immunogen:

Fusion protein of human MDM4

Full name:

MDM4, p53 regulator (MDM4), transcript variant 1

Synonyms:

HDMX; MDMX; MRP1

SwissProt:

O15151

WB Predicted band size:

55 kDa

WB Positive control:

Hela, SVT2, Jurkat, MCF-7 cell lysates

WB Recommended dilution:

200-1000