小鼠抗COX4I1单克隆抗体

Background:

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

Applications:

WB, FC, IF, IP, IHC

Name of antibody:

COX4I1

Immunogen:

Fusion protein of human COX4I1

Full name:

cytochrome c oxidase subunit 4I1

Synonyms:

COX4; COXIV; COX4-1; COXIV-1; COX IV-1

SwissProt:

P13073

IHC positive control:

Human colorectal carcinoma

IHC Recommend dilution:

25-50

WB Predicted band size:

20 kDa

WB Positive control:

Mouse skeletal muscel tissue and Jurkat cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

50-150

FC Recommend dilution:

50-100