小鼠抗CDKN1A单克隆抗体

Background:

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010].

Applications:

WB, IHC

Name of antibody:

CDKN1A

Immunogen:

Fusion protein of human CDKN1A

Full name:

cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 2

Synonyms:

CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1

SwissProt:

P38936

IHC positive control:

carcinoma of human thyroid tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

18 kDa

WB Positive control:

HepG2 and MDCK cell lysates

WB Recommended dilution:

200-1000