小鼠抗POLR2A单克隆抗体

Background:

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Applications:

WB, IHC

Name of antibody:

POLR2A

Immunogen:

Fusion protein of human POLR2A

Full name:

RNA polymerase II subunit A

Synonyms:

RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

SwissProt:

P24928

IHC positive control:

Human tonsil tissue and Human kidney tissue; Human lung cancer and Human pancreas tissue

IHC Recommend dilution:

200-500

WB Predicted band size:

217 KD

WB Positive control:

Jurkat, K562, 293T, Hela, MCF7, A549 and HepG2 cell lysates

WB Recommended dilution:

500-2000