小鼠抗POLR2E单克隆抗体

Background:

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].

Applications:

WB, IHC, IF, FC

Name of antibody:

POLR2E

Immunogen:

Fusion protein of human POLR2E

Full name:

polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)

Synonyms:

hRPB25; hsRPB5; RPABC1; RPB5; XAP4

SwissProt:

P19388

IHC positive control:

human breast tissue and carcinoma of human lung tissue; human pancreas tissue and carcinoma of human prostate tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

25 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000