兔抗KPNA2多克隆抗体

Background:

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants.

Applications:

ELISA, WB, IHC

Name of antibody:

KPNA2

Immunogen:

Fusion protein of human KPNA2

Full name:

karyopherin subunit alpha 2

Synonyms:

QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha

SwissProt:

P52292

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer

IHC Recommend dilution:

50-300

WB Predicted band size:

58 kDa

WB Positive control:

293T cell lysate

WB Recommended dilution:

1000-5000