小鼠抗H3C1单克隆抗体

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Applications:

WB, IHC

Name of antibody:

H3C1

Immunogen:

Fusion protein of human H3C1

Full name:

H3 clustered histone 1

Synonyms:

H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A

SwissProt:

P68431

IHC positive control:

Human ovarian cancer and Human breast cancer; Human gastric cancer and Human thyroid cancer

IHC Recommend dilution:

500-1000

WB Predicted band size:

15 kDa

WB Positive control:

Human left lung tissue lysate, Jurkat, 293T, K562, NIH/3T3, Hela and 231 cell lysates

WB Recommended dilution:

10000-50000