小鼠抗GRN单克隆抗体

Background:

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Applications:

WB, IHC

Name of antibody:

GRN

Immunogen:

Fusion protein of human GRN

Full name:

granulin precursor

Synonyms:

GEP; GP88; PEPI; PGRN; CLN11; PCDGF

SwissProt:

P28799

IHC positive control:

Human pancreas tissue and Human spleen tissue; Human endometrium tissue and Human appendix tissue

IHC Recommend dilution:

200-500

WB Predicted band size:

64 KD

WB Positive control:

A431, 293T, MCF7 and Hela cell lysates

WB Recommended dilution:

200-1000