小鼠抗DCPS单克隆抗体

Background:

Eukaryotic cells primarily utilize exoribonucleases and decapping enzymes to degrade their mRNA. DcpS is a scavenger pyrophosphatase that hydrolyzes the residual cap structure following 3' to 5' decay of an mRNA. Following mRNA degradation DcpS releases N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. The central histidine within the DcpS HIT motif is critical for decapping activity and defines the HIT motif as a new mRNA decapping domain, making DcpS the first member of the HIT family of proteins with a defined biological function. HIT proteins are homodimeric and contain two conserved 100-amino-acid HIT fold domains with independent active sites that are each sufficient to bind and hydrolyze cognate substrates.

Applications:

WB, IHC

Name of antibody:

DCPS

Immunogen:

Fusion protein of human DCPS

Full name:

decapping enzyme, scavenger (DCPS)

Synonyms:

ARS; DCS1; HSL1; HINT5; HINT-5; HSPC015

SwissProt:

Q96C86

IHC positive control:

carcinoma of human thyroid tissue and human endometrium tissue; human kidney tissue and human lymph node tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

39 kDa

WB Positive control:

HepG2, Hela, SVT2, COS7, PC12, MCF-7 cell lysates

WB Recommended dilution:

1000-5000