小鼠抗CBX5单克隆抗体

Background:

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Applications:

WB, IF, IHC

Name of antibody:

CBX5

Immunogen:

Fusion protein of human CBX5

Full name:

chromobox 5

Synonyms:

HP1; HP1A; HEL25

SwissProt:

P45973

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

200-400

WB Predicted band size:

22 kDa

WB Positive control:

Mouse brain tissue, COS7, C6, 3T3, HelaNE, 293T and MCF7 cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

150-300