小鼠抗AGO1单克隆抗体

Background:

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Applications:

WB, IHC

Name of antibody:

AGO1

Immunogen:

Fusion protein of human AGO1

Full name:

argonaute 1, RISC catalytic component

Synonyms:

Q99; EIF2C; hAgo1; EIF2C1; GERP95

SwissProt:

Q9UL18

IHC positive control:

Human embryonic cerebellum

IHC Recommend dilution:

50-150

WB Predicted band size:

97 KD

WB Positive control:

K562 cell, Human brain tissue lysates

WB Recommended dilution:

200-1000