兔抗CASP9(Phospho-Thr125)多克隆抗体

Background:

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade.

Applications:

WB, IHC

Name of antibody:

CASP9(Phospho-Thr125)

Immunogen:

Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.

Full name:

caspase 9, apoptosis-related cysteine peptidase

Synonyms：

MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

SwissProt:

P55211

IHC positive control:

Human lung carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

46 kDa

WB Positive control:

Hela cells lysates treated with TNF

WB Recommended dilution:

500-1000