兔抗CREBZF多克隆抗体

Background:

Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.

Applications:

ELISA, WB, IHC

Name of antibody:

CREBZF

Immunogen:

Synthetic peptide of human CREBZF

Full name:

CREB/ATF bZIP transcription factor

Synonyms：

ZF; SMILE

SwissProt:

Q9NS37

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human liver cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

37 kDa

WB Positive control:

Mouse brain tissue and PC3 cells

WB Recommended dilution:

500-2000