小鼠抗IPO5单克隆抗体

Background:

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Applications:

WB

Name of antibody:

IPO5

Immunogen:

Fusion protein of human IPO5

Full name:

importin 5

Synonyms:

IMB3; Pse1; imp5; KPNB3; RANBP5

SwissProt:

O00410

WB Predicted band size:

124 KD

WB Positive control:

A549, Hela, Jurkat, Raji cell, Mouse brain tissue lysates

WB Recommended dilution:

200-1000