小鼠抗EIF2S1单克隆抗体

Background:

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Applications:

WB, IF, IP

Name of antibody:

EIF2S1

Immunogen:

Fusion protein of human EIF2S1

Full name:

eukaryotic translation initiation factor 2 subunit alpha

Synonyms:

EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

SwissProt:

P05198

WB Predicted band size:

36 kDa

WB Positive control:

K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

100-200