小鼠抗E2F1单克隆抗体

Background:

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Applications:

WB, IF, IP

Name of antibody:

E2F1

Immunogen:

Fusion protein of human E2F1

Full name:

E2F transcription factor 1

Synonyms:

RBP3; E2F-1; RBAP1; RBBP3

SwissProt:

Q01094

WB Predicted band size:

47 kDa

WB Positive control:

C6, Raw264.7 and Hela cell lysates

WB Recommended dilution:

500-2000

IF positive control:

Hela cells

IF Recommend dilution:

50-100