小鼠抗CD22单克隆抗体

Background:

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Applications:

WB, IHC

Name of antibody:

CD22

Immunogen:

Fusion protein of human CD22

Full name:

CD22 molecule (CD22), transcript variant 1

Synonyms:

SIGLEC-2; SIGLEC2

SwissProt:

P20273

IHC positive control:

human lymph node tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

95 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000