小鼠抗ATP5B单克隆抗体

Background:

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Applications:

WB, IHC, IF, FC

Name of antibody:

ATP5B

Immunogen:

Fusion protein of human ATP5B

Full name:

ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B)

Synonyms:

ATPMB; ATPSB; HEL-S-271

SwissProt:

P06576

IHC positive control:

carcinoma of liver tissue

IHC Recommend dilution:

10-50

WB Predicted band size:

57 kDa

WB Positive control:

HepG2, Hela, HT-29, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000