兔抗CALR多克隆抗体

Background:

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.

Applications:

ELISA, WB, IHC

Name of antibody:

CALR

Immunogen:

Fusion protein of human CALR

Full name:

Calreticulin

Synonyms：

RO; CRT; SSA; cC1qR

SwissProt:

P27797

ELISA Recommended dilution:

2000-10000

IHC positive control:

Human ovarian cancer and Human gastric cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

48 kDa

WB Positive control:

Hela, 293T and NIH/3T3 cells

WB Recommended dilution:

1000-5000