兔抗PAK123(Ab-423402421)多克隆抗体

Background:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Applications:

WB, IHC

Name of antibody:

PAK1/2/3(Ab-423/402/421)

Immunogen:

Synthesized non-phosphopeptide derived from human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V).

Full name:

p21 protein (Cdc42/Rac)-activated kinase 1/2/3

Synonyms：

PAKalpha/PAK65; PAKgamma/bPAK; MRX30; MRX47; OPHN3; hPAK3; CDKN1A; PAK3beta

SwissProt:

Q13153/Q13177/O75914

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

61/58/62 kDa

WB Positive control:

NIH/3T3 cells lysate

WB Recommended dilution:

500-3000