兔抗MOB2多克隆抗体

Background:

MOB2 (Mps one binder kinase activator-like 2), also known as HCCA2 (Hepatocellular carcinoma-associated gene 2), is a 467 amino acid protein that belongs to the MOB1/phocein family. MOB2 is expressed in lung, spleen, brain, and fetal liver. It is highly expressed in hepatocellular carcinoma. MOB2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells. MOB2 is characterized by two N-glycosylation sites, six N-myristolylation sites, two Src homology 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade. MOB2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L(NDR2). It has been shown that MOB2 plays a critical role in cell cycle regulation. Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest. Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.

Applications:

ELISA, WB, IHC

Name of antibody:

MOB2

Immunogen:

Synthetic peptide of human MOB2

Full name:

MOB kinase activator 2

Synonyms:

HCCA2

SwissProt:

Q70IA6

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and Human thyroid cancer

IHC Recommend dilution:

20-100

WB Predicted band size:

27 kDa

WB Positive control:

Mouse liver tissue and Rat liver tissue lysates

WB Recommended dilution:

200-1000