兔抗ECD多克隆抗体

Background:

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).

Applications:

ELISA, WB, IHC

Name of antibody:

ECD

Immunogen:

Fusion protein of human ECD

Full name:

ecdysoneless cell cycle regulator

Synonyms:

GCR2; SGT1; HSGT1

SwissProt:

O95905

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human cervical cancer and Human thyroid cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

73 kDa

WB Positive control:

Jurkat and PC3 cell lysates

WB Recommended dilution:

200-1000