兔抗DFFA多克隆抗体

Background:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Applications:

ELISA, IHC

Name of antibody:

DFFA

Immunogen:

Fusion protein of human DFFA

Full name:

DNA fragmentation factor, 45kDa, alpha polypeptide

Synonyms：

DFF1, ICAD, DFF-45

SwissProt:

O00273

ELISA Recommended dilution:

1000-5000

IHC positive control:

Human cervical cancer and Human thyroid cancer

IHC Recommend dilution:

25-100