兔抗C1QTNF3多克隆抗体

Background:

C1qTNF3 (Complement C1q TNF-related protein 3/CTRP3; also CORS26 and cartonectin) is a 30-32 kDa, secreted member of the C1q and TNF-related protein (CTRP) superfamily of molecules. It is expressed by a wide variety of cells, including smooth muscle cells, fibroblasts, adipocytes, monocytes and proliferating chondrocytes. C1qTNF3 is an anti-inflammatory agent that apparently blocks LPS activation of mononuclear cells. It also has marked proliferative activity on diverse cell types such as vascular smooth muscle, chrondrocytes, and endothelium. Finally, C1qTNF3 is known to act on hepatocytes and suppress hepatocyte gluconeogenesis. Mature human C1qTNF3 is 224 amino acids (aa) in length (aa 23-246). It possesses an N-terminal collagen-like domain (aa 51-113) followed by a C-terminal globular region (aa 113-246). C1qTNF3 is monomeric when intracellular, but forms a 90 kDa homotrimer plus higher-order oligomer when secreted. There are at least two potential isoform variants. One is 40-42 kDa, glycosylated, and contains a 73 aa insertion after Glu28, while a second shows concurrent deletions of aa 46 69 and 82-105. The longer 40 kDa isoform is reported to form heterotrimers and oligomers with the standard 30 kDa isoform. This has the effect of protecting the standard isoform from proteolysis. Over aa 24-246, human C1qTNF3 shares 99% aa sequence identity with mouse C1qTNF3.

Applications:

ELISA, WB, IHC

Name of antibody:

C1QTNF3

Immunogen:

Fusion protein of human C1QTNF3

Full name:

C1q and tumor necrosis factor related protein 3

Synonyms：

CORS; CORCS; CTRP3; CORS26; C1ATNF3; CORS-26

SwissProt:

Q9BXJ4

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and human esophagus cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

27 kDa

WB Positive control:

Human colorectal cancer tissue

WB Recommended dilution:

200-1000