兔抗AKT1 (Phospho-Ser473)多克隆抗体

Background:

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Applications:

WB, IHC

Name of antibody:

AKT1 (Phospho-Ser473)

Immunogen:

Synthetic peptide of human AKT1 (Phospho-Ser473)

Full name:

v-akt murine thymoma viral oncogene homolog 1 (Phospho-Ser473)

Synonyms：

AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

SwissProt:

P31749

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

60 kDa

WB Positive control:

293 cells treated with EGF, Serum or both

WB Recommended dilution:

500-1000