小鼠抗PRKAA1单克隆抗体

Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Applications:

WB, IHC

Name of antibody:

PRKAA1

Immunogen:

Fusion protein of human PRKAA1

Full name:

Protein kinase, AMP-activated, alpha 1 catalytic subunit

Synonyms:

AMPK; AMPKa1

SwissProt:

Q13131

IHC positive control:

Human esophagus cancer and Human gastric cancer

IHC Recommend dilution:

100-500

WB Predicted band size:

64 kDa

WB Positive control:

Jurkat, 231 and NIH/3T3 cell lysates

WB Recommended dilution:

500-2000