小鼠抗PKM单克隆抗体

Background:

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Applications:

WB, IF, IP

Name of antibody:

PKM

Immunogen:

Fusion protein of human PKM

Full name:

pyruvate kinase, muscle

Synonyms:

PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

SwissProt:

P14618

WB Predicted band size:

58 kDa

WB Positive control:

HCT116, COS7, K562, A549 and Hela cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

3T3 cells

IF Recommend dilution:

250-500