小鼠抗SCFD1单克隆抗体

Background:

SCFD1 (sec1 family domain containing 1) is a 642 amino acid protein that belongs to the STXBP/unc-18/SEC1 family. Phosphorylated upon DNA damage, probably by Atm or ATR, SCFD1 is involved in vesicular transport between the endoplasmic reticulum (ER) and the Golgi. The SCFD1 protein plays a role in SNARE-pin assembly and Golgi to ER retrograde transport via its interaction with COG4. The SCFD1 protein also binds Syntaxin 5. The SCFD1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, mosquito, Drosophila, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana and rice, and maps to human chromosome 14q12. Deletion of a region of chromosome 14 that contains BF-1, PKC μ, SCFD1, Cochlin and SG2NA genes, is responsible for a condition resulting in severe mental retardation, epilepsy, microcephaly and Rett-like features.

Applications:

WB, IHC

Name of antibody:

SCFD1

Immunogen:

Fusion protein of human SCFD1

Full name:

sec1 family domain containing 1 (SCFD1), transcript variant 1

Synonyms:

C14orf163; RA410; SLY1; SLY1P; STXBP1L2

SwissProt:

Q8WVM8

IHC positive control:

adenocarcinoma of human ovary tissue and human pancreas tissue; adenocarcinoma of human endometrium tissue and human kidney tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

72 kDa

WB Positive control:

HepG2, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000