小鼠抗RFC4单克隆抗体

Background:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC, IF

Name of antibody:

RFC4

Immunogen:

Fusion protein of human RFC4

Full name:

replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1

Synonyms:

A1; RFC37

SwissProt:

P35249

IHC positive control:

human pancreas tissue and carcinoma of human pancreas tissue; human tonsil tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

40 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000