技术规格
| Background: | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]. | 
| Applications: | WB, IHC, IF | 
| Name of antibody: | RFC4 | 
| Immunogen: | Fusion protein of human RFC4 | 
| Full name: | replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 | 
| Synonyms: | A1; RFC37 | 
| SwissProt: | P35249 | 
| IHC positive control: | human pancreas tissue and carcinoma of human pancreas tissue; human tonsil tissue | 
| IHC Recommend dilution: | 30-150 | 
| WB Predicted band size: | 40 kDa | 
| WB Positive control: | HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates | 
| WB Recommended dilution: | 500-2000 | 
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