小鼠抗CDKN2A单克隆抗体

Background:

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Applications:

WB, IHC, FC

Name of antibody:

CDKN2A

Immunogen:

Fusion protein of human CDKN2A

Full name:

cyclin dependent kinase inhibitor 2A

Synonyms:

ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

SwissProt:

P42771

IHC positive control:

Human lung cancer and Human ovarian cancer

IHC Recommend dilution:

200-500

WB Predicted band size:

17 KD

WB Positive control:

293T and Hela cell lysates

WB Recommended dilution:

500-2000

FC Recommend dilution:

50-100