兔抗EEA1多克隆抗体

Background:

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking. The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes. The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin. Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion.

Applications:

ELISA, WB

Name of antibody:

EEA1

Immunogen:

Synthetic peptide of human EEA1

Full name:

early endosome antigen 1

Synonyms:

MST105; ZFYVE2; MSTP105

SwissProt:

Q15075

ELISA Recommended dilution:

5000-10000

WB Predicted band size:

163 kDa

WB Positive control:

Hela, 293T, RAW264.7 and NIH/3T3 cell lysates

WB Recommended dilution:

200-1000