兔抗DDB1多克隆抗体

Background:

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Applications:

ELISA, WB, IHC

Name of antibody:

DDB1

Immunogen:

Synthetic peptide of human DDB1

Full name:

DNA damage-binding protein 1

Synonyms：

XPE, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1

SwissProt:

Q16531

ELISA Recommended dilution:

500-5000

IHC positive control:

Human cervical cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

127 kDa

WB Positive control:

Human fetal small intestine, liver cancer and Lymphoma, 293T and A549 cells

WB Recommended dilution:

200-500