小鼠抗PPP4C单克隆抗体

Background:

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

Applications:

WB, IHC, IF

Name of antibody:

PPP4C

Immunogen:

Fusion protein of human PPP4C

Full name:

protein phosphatase 4 catalytic subunit

Synonyms:

PP4; PPX; PP-X; PP4C; PPH3; PPP4

SwissProt:

P60510

IHC positive control:

Human breast cancer

IHC Recommend dilution:

500-1000

WB Predicted band size:

35 kDa

WB Positive control:

Jurkat and Hela cell lysates

WB Recommended dilution:

200-1000

IF positive control:

Hela cells

IF Recommend dilution:

100-200