小鼠抗POLR3C单克隆抗体

Background:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Applications:

WB, IHC, IF

Name of antibody:

POLR3C

Immunogen:

Fusion protein of human POLR3C

Full name:

polymerase (RNA) III (DNA directed) polypeptide C (62kD) (POLR3C), transcript variant 1

Synonyms:

RPC3; RPC62

SwissProt:

Q9BUI4

IHC positive control:

human bladder tissue and carcinoma of human thyroid tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

61 kDa

WB Positive control:

Hela, SVT2, A549, COS7, Jurkat, MDCK, MCF-7 cell lysates

WB Recommended dilution:

500-2000