小鼠抗RAPGEF1单克隆抗体

Background:

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC, IF

Name of antibody:

RAPGEF1

Immunogen:

Fusion protein of human RAPGEF1

Full name:

Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2

Synonyms:

C3G; GRF2

SwissProt:

Q13905

IHC positive control:

human breast tissue and carcinoma of human thyroid tissue; carcinoma of human prostate tissue and adenocarcinoma of human colon tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

121 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000