兔抗HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220)多克隆抗体

Background:

Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.

Applications:

WB, IHC

Name of antibody:

HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220)

Immunogen:

Synthetic peptide of human HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220)

Full name:

HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220)

Synonyms：

HD4/HD5/HD9

SwissProt:

P56524/Q9UQL6/Q9UKV0

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

124 kDa; 140 kDa

WB Positive control:

293 cells untreated or treated with EGF

WB Recommended dilution:

500-1000