反应种属: Human
克隆类型: rabbit polyclonal
技术规格
Background: |
Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. |
Applications: |
WB, IHC |
Name of antibody: |
HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) |
Immunogen: |
Synthetic peptide of human HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) |
Full name: |
HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) |
Synonyms: |
HD4/HD5/HD9 |
SwissProt: |
P56524/Q9UQL6/Q9UKV0 |
IHC positive control: |
Human breast carcinoma |
IHC Recommend dilution: |
50-100 |
WB Predicted band size: |
124 kDa; 140 kDa |
WB Positive control: |
293 cells untreated or treated with EGF |
WB Recommended dilution: |
500-1000 |

