兔抗IFIT3多克隆抗体

Background:

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

Applications:

ELISA, WB, IHC

Name of antibody:

IFIT3

Immunogen:

Fusion protein of human IFIT3

Full name:

interferon induced protein with tetratricopeptide repeats 3

Synonyms:

P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; cig41; CIG-49; GARG-49

SwissProt:

O14879

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human cervical cancer and Human tonsil

IHC Recommend dilution:

20-100

WB Predicted band size:

56 kDa

WB Positive control:

HepG2 cell lysate

WB Recommended dilution:

200-1000