兔抗HLA-DPA1多克隆抗体

Background:

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Applications:

ELISA, WB, IHC

Name of antibody:

HLA-DPA1

Immunogen:

Synthetic peptide of human HLA-DPA1

Full name:

major histocompatibility complex, class II, DP alpha 1

Synonyms:

PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A

SwissProt:

P20036

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human tonsil

IHC Recommend dilution:

40-200

WB Predicted band size:

29 kDa

WB Positive control:

Raji cell, Human spleen tissue lysates

WB Recommended dilution:

500-2000