小鼠抗MED27单克隆抗体

Background:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.

Applications:

WB

Name of antibody:

MED27

Immunogen:

Fusion protein of human MED27

Full name:

mediator complex subunit 27

Synonyms:

MED3; CRSP8; CRAP34; CRSP34; TRAP37

SwissProt:

Q6P2C8

WB Predicted band size:

35 KD

WB Positive control:

HepG2, K562, Hela, MCF7, Jurkat, A549, 293T cell lysates

WB Recommended dilution:

500-2000