小鼠抗MAP2K3单克隆抗体

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC, IF

Name of antibody:

MAP2K3

Immunogen:

Fusion protein of human MAP2K3

Full name:

mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B

Synonyms:

MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2

SwissProt:

P46734

IHC positive control:

adenocarcinoma of human ovary tissue and adenocarcinoma of human endometrium tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

39 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, MCF-7 cell lysates

WB Recommended dilution:

500-2000