小鼠抗APEX1单克隆抗体

Background:

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC

Name of antibody:

APEX1

Immunogen:

Fusion protein of human APEX1

Full name:

APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1

Synonyms:

APE; APE1; APEN; APEX; APX; HAP1; REF1

SwissProt:

P27695

IHC positive control:

human colon tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

36 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, MCF-7 cell lysates

WB Recommended dilution:

1000-5000