兔抗AKT1/AKT2/AKT3 (phospho-Tyr315/316/312)多克隆抗体

Background:

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Applications:

WB, IHC, IF

Name of antibody:

AKT1/AKT2/AKT3 (phospho-Tyr315/316/312)

Immunogen:

Synthetic peptide of human AKT1/AKT2/AKT3 (phospho-Tyr315/316/312)

Full name:

AKT1/AKT2/AKT3 (phospho-Tyr315/316/312)

SwissProt:

P31749

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

60 kDa

WB Positive control:

HepG2 cells treated with EGF

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200