小鼠抗PYCARD单克隆抗体

Background:

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Applications:

WB, IHC, FC

Name of antibody:

PYCARD

Immunogen:

Fusion protein of human PYCARD

Full name:

PYD and CARD domain containing (PYCARD), transcript variant 1

Synonyms:

ASC; CARD5; TMS; TMS-1; TMS1

SwissProt:

Q9ULZ3

IHC positive control:

carcinoma of human pancreas tissue and human tonsil tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

22 kDa

WB Positive control:

A549, MCF-7 cell lysates

WB Recommended dilution:

200-1000